|
This study aims at producing red pigment effectively by using protoplast fusion, Auxotrophic mutants of M, anka, 4478-27-37(Thi^-, Met^-), 4478-27-62(Thi^-, Arg^-) were derived from M. anka, 4478-27. M. anka, 6540-185-24 (Pan^-, Leu^-) and M. anka, 6540-185-72 (Arg^-) were derived from M. anka, 6540-185. The optimal conditions of protoplast fromation were at time by using mixtures of chitinase (100㎎/㎖), cellulase (5㎎/㎖) and β-glucuronidase (5㎎/㎖). The protoplast of those auxotrophic mutants were fused effectively, in the solution of 30% PEG 6,000, 0.01M-CaCl₂, 0.05M-glycine, pH 6.0. Fusion frequencies were 0.70%-0.85%. Fusants, No. 14 and 42 produced highest red pigment among them.
|